Journal: Clinical and Experimental Medicine

Article Title: Investigating the role of circulating tumor cells in gastric cancer: a comprehensive systematic review and meta-analysis

doi: 10.1007/s10238-024-01310-6

Figure Lengend Snippet: Overall estimates of meta-analysis conducted on the correlation between incidence rate of CTCs and the prognosis of overall survival of PFS

Article Snippet: The result of prognostic role of CTCs detected by Cell Search and cytological methods showed that detection methods whether based on epithelial markers or mesenchymal markers may be appropriate for a population of CTCs in GC.

Techniques: Marker

Journal: BMC Pulmonary Medicine

Article Title: Circulating tumor cells prior to initial treatment is an important prognostic factor of survival in non-small cell lung cancer: a meta-analysis and system review

doi: 10.1186/s12890-019-1029-x

Figure Lengend Snippet: Evaluation of PFS with high or low abundance of CTCs by using meta-analysis ( a - c ) and systematic review ( d ). a Higher abundance of CTCs predicts shorter PFS of NSCLC. b Stratified study found that the differences of heterogeneity among tumor stages are quite small(I 2 = 0%). c Stratified study found that the differences of heterogeneity among various detect methods of CTCs are moderate(I 2 = 36.1%). d Systematic review of PFS (months) data indicated higher abundance of CTCs are asscociated with shorter PFS

Article Snippet: As Cell search, EpCAM (−) CTCs cannot be detected by MACS.

Techniques:

Journal: BMC Pulmonary Medicine

Article Title: Circulating tumor cells prior to initial treatment is an important prognostic factor of survival in non-small cell lung cancer: a meta-analysis and system review

doi: 10.1186/s12890-019-1029-x

Figure Lengend Snippet: Evaluation of OS with high or low abundance of CTCs by using meta-analysis ( a - c ) and systematic review ( d ). a . Higher abundance of CTCs had not significant predict power of prognosis of lung cancer cases. b Stratified analysis showed the differences of tumor stages in different cohorts lead to heterogeneity(I 2 = 81.5%). c Stratified analysis showed various detect methods in different cohorts lead to heterogeneity (I 2 = 79.0%). d Systematic review of OS (months) data indicated higher abundance of CTCs are associated with shorter OS

Article Snippet: As Cell search, EpCAM (−) CTCs cannot be detected by MACS.

Techniques:

Journal: BMC Pulmonary Medicine

Article Title: Circulating tumor cells prior to initial treatment is an important prognostic factor of survival in non-small cell lung cancer: a meta-analysis and system review

doi: 10.1186/s12890-019-1029-x

Figure Lengend Snippet: Summary of the published studies included in the meta-analysis and system analysis

Article Snippet: As Cell search, EpCAM (−) CTCs cannot be detected by MACS.

Techniques: Biomarker Discovery

Journal: Journal of Extracellular Vesicles

Article Title: Monitoring HSP70 exosomes in cancer patients’ follow up: a clinical prospective pilot study

doi: 10.1080/20013078.2020.1766192

Figure Lengend Snippet: HSP70 exosomes compared to CTCs to discriminate metastatic from non-metastatic patients. ROC curve analysis for the detection level of HSP70 exosomes (left panel) or circulating tumour cells (CTCs, right panel), AUC = 0.8968, CI95% = [0.7387–1], p < 0.0001 compared to 0.7857, CI95% = [0.5873–0.9842], p = 0.0048.

Article Snippet: Next, we compared circulating HSP70 exosomes with CTCs quantified by Cell-Search (a current FDA-approved approach), as predictors of tumour progression.

Techniques:

Journal: Micromachines

Article Title: Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

doi: 10.3390/mi15060706

Figure Lengend Snippet: Label-dependent microfluidic methods for CTCs isolation.

Article Snippet: , , Microfluidic ratchet mechanism , CTCs captured with 25 times more than traditional Cell Search systems. , Relatively low throughput. , UM-UC-13 , 1 , >90% , [ ] .

Techniques: Isolation, Generated, Adsorption, High Throughput Screening Assay, Activity Assay, Filtration, Magnetic Beads, Immunofluorescence, Immunostaining

Journal: Micromachines

Article Title: Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

doi: 10.3390/mi15060706

Figure Lengend Snippet: Typical immunocapture microfluidic device. ( A ) Using aptamer cocktail to enrich CTCs in patients with lung cancer; reproduced from Reference , with a permission from Small . ( B ) Herringbone microfluidic probe. (a) The schematic shows the working process of HB-MFP, in which blood is injected through the central aperture and exits through the peripheral aperture. (b) The tip surface of 3D-printed HB-MFP. (c,d) Specifically showing the surface structure in (b); reproduced from Reference , with permission from Advanced Materials Technologies . ( C ) Scheme shows the structural composition and working principle of the Ap Octopus Chip. The chip captures CTCs, using the synergistic manner of multivalent aptamers formed by AuNP-SYL3C and a triangular DLD array design. In the sequencing curves, green, red, blue and black represent adenine (A), thymine (T), cytosine (C) and guanine (G); respectively; reproduced from Reference , with permission from Angewandte Chemie International Edition . ( D ) The process of capturing CTCs using aptamer modified AuNP surfaces; reproduced from Reference , with permission from ACS Nano .

Article Snippet: , , Microfluidic ratchet mechanism , CTCs captured with 25 times more than traditional Cell Search systems. , Relatively low throughput. , UM-UC-13 , 1 , >90% , [ ] .

Techniques: Injection, Sequencing, Modification

Journal: Micromachines

Article Title: Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

doi: 10.3390/mi15060706

Figure Lengend Snippet: Label-independent microfluidic methods for CTCs isolation.

Article Snippet: , , Microfluidic ratchet mechanism , CTCs captured with 25 times more than traditional Cell Search systems. , Relatively low throughput. , UM-UC-13 , 1 , >90% , [ ] .

Techniques: Isolation, High Throughput Screening Assay, Shear, Sample Prep

Journal: Micromachines

Article Title: Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

doi: 10.3390/mi15060706

Figure Lengend Snippet: ( A ) Schematic illustration of the double spiral microchannel to achieve cell focusing. The diagram is labeled with the inlets, outlets, and the direction of flow. The numbers 1, 2, 3 represent the flow state of cells in three different positions of the microchannel; reproduced from Reference , with permission from Frontiers in Bioengineering and Biotechnology . ( B ) Schematic illustration of the working principle of the CEA microchannel; reproduced from Reference , with permission from Analytical Chemistry . ( C ) Isolation of CTCs by a serpentine microchannel; reproduced from Reference , with permission from Micro and Nano Engineering . ( D ) Schematic illustration of the microfluidic device and working principle of inertial migration in microfluidic channel; reproduced from Reference , with permission from Microsystems & Nanoengineering . ( E ) Schematic illustration of p-MOFF device. (a) Working principle diagram. (b) Microfiltration microscopy images; reproduced from Reference , with permission from Biosensors and Bioelectronics .

Article Snippet: , , Microfluidic ratchet mechanism , CTCs captured with 25 times more than traditional Cell Search systems. , Relatively low throughput. , UM-UC-13 , 1 , >90% , [ ] .

Techniques: Labeling, Isolation, Migration, Microscopy

Journal: Micromachines

Article Title: Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

doi: 10.3390/mi15060706

Figure Lengend Snippet: ( A ) Schematic illustration of the working principle of integrated microfluidic device. The device consists of two parts. The Vortex HT Chip can achieve high-throughput enrichment of CTCs, and the impedance chip can count the collected CTCs; reproduced from Reference , with permission from Cytometry Part A . ( B ) Schematic illustration of the working principle of the orthogonal vortex chip. Schematic diagram of the working principle of the vortex chip with orthogonal reversal. Enrichment of CTCs by using the generated orthogonal vortex and Dean drag force; reproduced from Reference , with permission from Analytica Chimica Acta . ( C ) TO DLD Chip Device Isolation CTCs. (a) The schematic illustration of the wide TO DLD chip. (b) The PDMS microchannel in TO DLD chip; reproduced from Reference , with permission from the Journal of Chromatography A . ( D ) Integrated Microfluidic Chip for isolation of CTCs. (a) Schematic illustration of the microfluidic chip. (b) Working schematic diagram for separating CTCs according to size inside a microchannel. (c,d) Triangular DLD arrays within the microfluidic channel for separating cells of different sizes. Cancer cells (yellow), red blood cells (red), and white blood cells (white); reproduced from Reference , with permission from Advanced Biosystems .

Article Snippet: , , Microfluidic ratchet mechanism , CTCs captured with 25 times more than traditional Cell Search systems. , Relatively low throughput. , UM-UC-13 , 1 , >90% , [ ] .

Techniques: High Throughput Screening Assay, Cytometry, Generated, Isolation, Chromatography

Journal: Micromachines

Article Title: Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

doi: 10.3390/mi15060706

Figure Lengend Snippet: ( A ) The high-throughput taSSAW device for CTCs isolation. (a) Illustration of cell isolation in the taSSAW device. (b) Schematic illustration of the principle of taSSAW-based cell isolation. (c) Actual size image of the tasSAW cell isolation device; reproduced from Reference , with permission from the Proceedings of the National Academy of Sciences . ( B ) Schematic illustration of an ultra-compact acoustofluidic device based on np-TSAW for enrichment of CTCs; reproduced from Reference , with permission from Analytica Chimica Acta . ( C ) Schematic illustration of CTCs isolation based on multi-stage surface acoustic waves; reproduced from Reference , with permission from Sensors and Actuators B: Chemical . ( D ) Microfluidic system based on optically induced dielectrophoretic (ODEP); reproduced from Reference , with permission from Scientific Reports . ( E ) Schematic illustration of a microdevice based on LFFF-DEP; reproduced from Reference , with permission from the Journal of Chromatography B . ( F ) Microfluidic device for impedance detection and dielectrophoresis integration. (a) Structural diagram of the device. (b) Cell Flow and DEP trapping. (c) Identification of target cells by impedance; reproduced from Reference , with permission from Biosensors and Bioelectronics .

Article Snippet: , , Microfluidic ratchet mechanism , CTCs captured with 25 times more than traditional Cell Search systems. , Relatively low throughput. , UM-UC-13 , 1 , >90% , [ ] .

Techniques: High Throughput Screening Assay, Isolation, Cell Isolation, Chromatography

Journal: Micromachines

Article Title: Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

doi: 10.3390/mi15060706

Figure Lengend Snippet: ( A ) Negative enrichment of CTCs based on GASI-ship; reproduced from Reference , with permission from Analytical Chemistry . ( B ) Monolithic chip for isolation of CTCs; reproduced from Reference , with permission from Scientific Reports . ( C ) Immunomagnetic negative enrichment coupled with flow cytometry to isolate CTCs; reproduced from Reference , with permission from Cancer . ( D ) Microfluidic Cell Concentrator for Negative Enrichment of CTCs. (a) 3D schematic diagram of the device. (b) CTCs collection process; reproduced from Reference , with permission from Methods . ( E ) μ-MixMACS chip. (a) Schematic illustration of the structure of the chip. (b) Illustration of the working principle of this chip to capture CTCs; reproduced from Reference , with permission from Sensors and Actuators B: Chemical . ( F ) Two-stage microfluidic chip. (a) Leukocyte-depleted μ-MACS chips. (b) Illustration of GASI chip; reproduced from Reference , with permission from Biosensors and Bioelectronics .

Article Snippet: , , Microfluidic ratchet mechanism , CTCs captured with 25 times more than traditional Cell Search systems. , Relatively low throughput. , UM-UC-13 , 1 , >90% , [ ] .

Techniques: Isolation, Flow Cytometry

Journal: Micromachines

Article Title: Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

doi: 10.3390/mi15060706

Figure Lengend Snippet: ( A ) Schematic illustration of microfluidic single-cell capture principle; reproduced from Reference , with permission from Scientific Reports . ( B ) Single-cell RNA sequencing of CTCs using Hydro-Seq.; reproduced from Reference , with permission from Nature Communications . ( C ) Schematic illustration of the working principle of capturing and releasing single CTCs based on the photoelectrochemical platform; reproduced from Reference , with permission from Nature Communications . ( D ) Schematic of an integrated microvalve chip capable of capturing at the single-cell level; reproduced from Reference , with permission from Talanta . ( E ) Schematic illustration of pipette working principle based on microfluidic cell isolation technology; reproduced from Reference , with permission from Scientific Reports . ( F ) Working principle of hand-held and integrated single-cell pipettes; reproduced from Reference , with permission from the Journal of the American Chemical Society . ( G ) Single-cell isolation device based on lateral magnetophoretic isolation and microfluidic dispensing; reproduced from Reference , with permission from Analytical Chemistry .

Article Snippet: , , Microfluidic ratchet mechanism , CTCs captured with 25 times more than traditional Cell Search systems. , Relatively low throughput. , UM-UC-13 , 1 , >90% , [ ] .

Techniques: RNA Sequencing, Transferring, Cell Isolation, Single-cell Isolation, Isolation

Journal: Micromachines

Article Title: Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review

doi: 10.3390/mi15060706

Figure Lengend Snippet: Clinical applications of CTCs in cancer.

Article Snippet: , , Microfluidic ratchet mechanism , CTCs captured with 25 times more than traditional Cell Search systems. , Relatively low throughput. , UM-UC-13 , 1 , >90% , [ ] .

Techniques: Immunostaining, Clinical Proteomics, Immunofluorescence, Staining, Expressing, Flow Cytometry

Journal: Neuro-Oncology

Article Title: Liquid biopsy in central nervous system metastases: a RANO review and proposals for clinical applications

doi: 10.1093/neuonc/noz012

Figure Lengend Snippet: Studies on CSF circulating tumor cells (CTCs) versus CSF cytology in LM

Article Snippet: NCT01645839 , 144 patients with LM from breast cancer , Phase III trial • Arm A: Standard systemic treatment without intrathecal liposomal ARA-C • Arm B: Standard systemic treatment with intrathecal liposomal ARA-C , CSF , CTCs using Veridex technique , Neurological PFS , - Clinical PFS (Montreal Cognitive Assessment Scale [MOCA] score) - Cytological PFS - Radiological PFS - OS - Tolerability of liposomal ARA-C - Research and quantification of tumor cells in the CSF for the diagnosis and monitoring of meningeal metastasis of breast cancer.

Techniques: Biomarker Discovery

Journal: Neuro-Oncology

Article Title: Liquid biopsy in central nervous system metastases: a RANO review and proposals for clinical applications

doi: 10.1093/neuonc/noz012

Figure Lengend Snippet: Ongoing clinical studies on CNS metastases including liquid biopsy

Article Snippet: NCT01645839 , 144 patients with LM from breast cancer , Phase III trial • Arm A: Standard systemic treatment without intrathecal liposomal ARA-C • Arm B: Standard systemic treatment with intrathecal liposomal ARA-C , CSF , CTCs using Veridex technique , Neurological PFS , - Clinical PFS (Montreal Cognitive Assessment Scale [MOCA] score) - Cytological PFS - Radiological PFS - OS - Tolerability of liposomal ARA-C - Research and quantification of tumor cells in the CSF for the diagnosis and monitoring of meningeal metastasis of breast cancer.

Techniques: Biomarker Discovery, Clinical Proteomics, Next-Generation Sequencing, Mutagenesis, Control, Comparison, Expressing, Sampling